SafePath Campylobacter Immunoassay Kit 96-well
Product Code: CYF-96
The SafePath Campylobacter assay is an enzyme-linked immunosorbent
assay (ELISA) that may be used to screen food products for the presence of
thermophilic Campylobacter antigen.
Infection by thermophilic Campylobacter species is a leading cause of human
gastroenteritis. Of the various species of Campylobacter, C. jejuni, C. coli and
C. lari, are the species most often associated with human illness.
Campylobacter are often passed to humans through the handling or
consumption of contaminated food, particularly foods of animal origin. Recently,
human infection with Campylobacter has been implicated in the induction of
Guillain-Barré Syndrome (GBS) and reactive arthritis. GBS is a debilitating and
potentially fatal neurological disease that produces paralysis.
Campylobacter species are gram negative, motile curved or spiral rods that
require highly specialized growth conditions. Typical cultivation entails
pre-enrichment and enrichment steps in broth, followed by isolation on a
selective solid medium. Of particular importance in the cultivation of
Campylobacter is the requirement for a microaerobic atmosphere. Cultivation of
thermophilic Campylobacter species from foods involves multiple media steps,
and thus many days.
The SafePath Campylobacter Microwell ELISA allows rapid testing (less than
an hour) of the enrichment broth, thus eliminating the need for the plating and
identification steps used in the standard methods. The SafePath
Campylobacter Microwell ELISA reduces time to result by several days.
Principle of the Test
The SafePath Campylobacter ELISA is a double antibody (sandwich) ELISA
utilizing specific anti-Campylobacter antibodies coated to microwells. After
addition of the sample and the enzyme conjugate, a positive reaction
(indicating the presence of Campylobacter antigen) produces a deep blue color.
Addition of the Stop Solution ends the assay and turns the blue color to yellow.
The results may be read visually or with an ELISA reader.