SafePath Cryptosporidium Immunoassay Kit 96-well
Product Code: CPA-96

Intended Use

This ELISA is a qualitative procedure for the determination of Cryptosporidium
antigen in animal feces.

Principle of Procedure

During the first incubation, Cryptosporidium antigens present in the sample are
captured by antibodies attached to the test wells. The second incubation adds
an additional anti-Cryptosporidium antibody that "sandwiches" the antigen.
The next incubation adds an anti-goat antibody conjugated to peroxidase
(HRP). After washing to remove any unbound enzyme, a chromogen is added
which develops a blue color in the presence of the enzyme complex and
peroxide. The Stop Solution ends the reaction, turning the blue assay color to
yellow.

Included with each kit

Test strips: Microwells containing anti-Cryptosporidium rabbit polyclonal
antibodies - 96 test wells.

Test strip holder: One (1).

Reagent 1: One (1) bottle containing 11 ml of anti-Cryptosporidium goat
polyclonal antibody with preservative.

Reagent 2: One (1) bottle containing 11 ml of anti-goat peroxidase with
preservative.

Positive control: One (1) vial containing 2 ml of Cryptosporidium oocysts in
buffer with preservative.

Negative control: One (1) vial containing 2 ml of a Cryptosporidium negative
sample in buffer with preservative.

Chromogen: One (1) bottle containing 11 ml of the chromogen
tetramethylbenzidine (TMB)

Wash Concentrate 20X: Two (2) bottles containing 25 ml of concentrated
buffer and surfactant with preservative.

Stop solution: One (1) bottle containing 11 ml of 1M phosphoric acid