SafePath Listeria Immunoassay Kit 96-well
Product Code: LIS-96

Intended Use

The SafePath Listeria assay is an enzyme-linked immunosorbent assay
(ELISA) that may be used to screen food products for the presence of Listeria
antigen.

Summary

The genus Listeria is comprised of six species (L. monocytogenes, L. ivanovii, L.
innocua, L. welshimeri, L. seegligeri and L. grayi). Of these six species, only L.
monocytogenes has been consistently associated with human illness although
presence of the other species may indicate that contamination with L.
monocytogenes is possible. L. monocytogenes (LM) is widespread in nature and
has been isolated from diverse sources such as soil, vegetation, water and
marine sediments. Foodstuff's found contaminated with LM include raw lobster,
raw and cooked shrimp, various vegetables (especially those grown
organically) and raw and smoked fish. Lately, the greatest threat to the
consumer has been the outbreaks of Listeria from ready-to-eat food products
such as hot dogs and cold cuts.

The SafePath Listeria ELISA is a rapid and reliable test, which significantly
reduces the time required to screen foods for the presence of Listeria. The
assay can detect LM, L. innocua and L. welshimeri. Primary enrichment cultures
grown for 24 to 48 hours can be tested in less than one hour, allowing
ELISA-negative product to be released sooner than with standard methods.
Presumptively positive ELISA samples can be cultured further for confirmation
by standard methods.

Principle of the Test

The SafePath Listeria ELISA is a double antibody (sandwich) ELISA utilizing
specific anti-Listeria antibodies coated to microwells. After addition of the
sample and the enzyme conjugate, a positive reaction (indicating the presence
of Listeria antigen) produces a deep blue color. Addition of the Stop Solution
ends the assay and turns the blue color to yellow. The results may be read
visually or with an ELISA reader.