SafePath Milk Residue Immunoassay Kit
Product Code: Beta-48

For the detection of Milk Residues in food, food products and related samples.
A 48 well semi-quantiative test kit containing controls at negative, 1ppm and
5ppm.
Background Information
The SafePath Laboratories Milk Residue Microwell Immunoassay kit is designed
to provide a sensitive and convenient method for screening samples for Milk
residues in food and related samples.
Beta-Lactoglobulin is the major whey protein in ruminants and pigs. Human
infants can develop an allergenic response to cow milk proteins.
Beta-Lactoglobulin is the primary antigenic component that stimulates the
immune hypersensitivity response in the infant. Symptoms are similar to
lactose intolerance.
During the first incubation, Beta Lactoglobulin in the sample is captured by
antibodies attached to the wells.
In the second incubation, a peroxidase conjugated anti-Beta Lactoglobulin
polyclonal antibody binds to the captured Beta Lactoglobulin.
After washings to remove unbound enzyme conjugate, a chromogen is added
which develops a blue color in the presence of the enzyme complex and
peroxide.
The stop solution ends the reaction and turns the blue color to yellow.
Samples are compared to a Positive Control which has been set as a screening
level in this assay.
Results may be determined visually, or by using a microplate reader.
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