SafePath Salmonella Immunoassay Kit 960-well
Product Code: SAL-960

Intended Use

A rapid, 55 minute test for Salmonella antigen in food or environmental samples.

Summary

Salmonella is a significant cause of human food-borne illness. Salmonella occurs
in a wide variety of foods, however poultry meat and poultry products are
recognized as a frequent source of human exposure to this pathogen. Several
countries have targeted Salmonella in recent food safety initiatives. It is
anticipated that limiting the contamination of food products, animal feeds and
animal environments with Salmonella will reduce the economic and health
impacts of food-borne infections in people. Although many methods exist for
detecting Salmonella in foods, most are cumbersome, time consuming and not
applicable to some production situations. Screening by the SafePath Salmonella
assay rapidly distinguishes samples that are free of Salmonella from those that
are presumptively positive and require confirmatory testing.

Principle of Procedure

Test samples are cultured as recommended and a portion of the broth culture is
heated briefly to release the Salmonella antigen and to inactivate the bacteria
before being added to the microwells. During the first incubation, Salmonella LPS
antigen in the sample is captured by antibodies attached to the wells. In the
second incubation, a peroxidase conjugated anti-Salmonella monoclonal antibody
binds to the captured Salmonella antigen. After washings to remove unbound
enzyme conjugate, a chromogen is added which develops a blue color in the
presence of the enzyme complex and peroxide. The stop solution ends the
reaction and turns the blue color to yellow.

Test Procedure

1. Break off number of wells needed (number of samples plus 2 for controls) and
place in strip holder.
2. Add 100 ul of the negative control to well #1 and 100 ul of positive control to
well #2 (use both as undiluted).
3. Add 100 ul of the test supernatant to the appropriate test well.
4. Incubate at room temperature for 60 minutes, then wash.*
5. Add 2 drops of Enzyme Conjugate to each well.
6. Incubate at room temperature for 30 minutes, then wash.
7. Rinse each well once with DI water.
8. Add 1 drop of Chromogen and 1 drop of Substrate to each well. Mix wells by
gently tapping strip holder.
9. Incubate at room temperature for 10 minutes.
10. Add 2 drops of Stop Solution to each well. Mix wells by tapping strip holder.
11. Read results visually or on a spectrophotometer using a bichromatic reading,
with the filters set at 450nm and 620-650nm. Zero the reader on air.

This Kit Contains 960 wells.